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无磷洗衣粉对喜水植物种子萌发及生长的影响
刘蓉1, 谈昊天2, 郑思2, 明凤1
1.上海师范大学 生命科学学院, 上海 200234;2.上海中学, 上海 200237
摘要:
选用水稻、碗莲及梭鱼草为实验材料,研究了无磷洗衣粉对于喜水植物生长及其种子萌发的影响.实验设5个处理,即清水对照、2种低浓度无磷洗衣粉溶液(质量分数0.02%,0.05%)和2种高浓度无磷洗衣粉溶液(质量分数0.10%,0.30%).研究发现:(1)随着无磷洗衣粉浓度的增大,碗莲和水稻种子发芽率降低,芽长缩短,根系生长停滞、萎缩,根尖变黑,发育性状变差;(2)随着无磷洗衣粉溶液浓度增大,梭鱼草植株高度变矮,叶片发黄,健康程度下降,生长受到抑制,植株有枯萎迹象.选取2个与水稻根部生长发育有关的基因OsDREB2BOsARF12,发现经过无磷洗衣粉溶液处理的水稻幼苗根系中,这两个基因的表达量明显低于清水组.研究结果表明:无磷洗衣粉对喜水植物生长及其种子萌发有抑制作用,无磷洗衣粉并非是含磷洗衣粉的完美替代,依然对生态有着副作用,需要进一步开发环境友好型替代产品.
关键词:  无磷洗衣粉  喜水植物  萌发率  生长  生理指标
DOI:10.20192/j.cnki.JSHNU(NS).2024.06.008
分类号:Q947.9
基金项目:上海市科技兴农项目(2022-02-08-00-12-F01161)
The effect of nonphosphorus washing powder on the germination and growth of hydrophilous plant seeds
LIU Rong1, TAN Haotian2, ZHENG Si2, MING Feng1
1.College of Life Sciences, Shanghai Normal University, Shanghai 200234, China;2.Shanghai High School, Shanghai 200237, China
Abstract:
In order to explore the effects of nonphosphorus washing powder on different hydrophilous plants, rice (Oryza sativa L.), water lily (Nelumbo noucifera Gaertn) and Pontederia cordata L. were selected as experimental materials to study the effects of nonphosphorus washing powder on the growth and seed germination of hydrophilous plants. Five treatments were set up in the experiment, namely, clean water control, two low concentration solution (mass fraction 0.02%, 0.05%) and two high concentration solution (mass fraction 0.10%, 0.30%) of nonphosphorus washing powder. The results showed that: (1) With the increase of nonphosphorus washing powder concentration, the germination rate of water lily and rice seeds decreased, the bud length shortened, the root growth stagnated and shrank, the root tip turned black, and the development characteristics became worse; (2) With the increase of the concentration of phosphate-free washing powder solution, the height of the plant became shorter, the leaves turned yellow, the health of the plant declined, the growth was inhibited, and the plant showed signs of wilting. Two genes related to the growth and development of rice roots, OsDREB2B and OsARF12, were selected. It was found that the expression levels of these two genes in the roots of rice seedlings treated with nonphosphate washing powder solution were significantly lower than those in the clean water group. The results show that nonphosphorus washing powder can inhibit the growth and seed germination of water-loving plants. Nonphosphorus washing powder is not a perfect substitute for phosphorus-containing washing powder, but still has side effects on the ecology. So it is necessary to further develop environmentally friendly alternative products.
Key words:  nonphosphate laundry detergent  hydrophilous plant  germination rate  growth  physiological index