| 摘要: |
| 基于花药转录组数据,分析拟南芥tek/ms188双突变体花药发育过程中花粉壁相关基因的调控模式.与野生型转录组数据对比,在tek/ms188突变体中共检测到了1 588个表达差异基因,其中,211个上调（13.3%）,1 377个下调（86.7%）.GO富集分析共富集到1 139个差异基因,有176个上调（15.5%）,963个下调（84.5%）.差异基因主要集中在生物过程（46.6%）中的花粉管生长与细胞壁调控过程.花粉管聚类中,大量的下调基因主要集中在细胞壁组成中.同时,KEGG分析发现,在戊糖和葡萄糖酸盐的代谢通路中,存在特异表达UDP-糖-焦磷酸化酶（USP）.本研究的初步结果表明：花粉管壁发育的相关基因也参与了花粉壁发育.通过分析tek/ms188双突变体对拟南芥雄性育性的影响,深入分析植物雄性生殖发育过程,并为第三代杂交水稻的研究进程提供一定的理论支持. |
| 关键词: 转录组分析 花粉管发育 花粉壁发育 TEK MS188 |
| DOI：10.20192/j.cnki.JSHNU（NS）.2024.05.010 |
| 分类号:Q945.5 |
| 基金项目:国家自然科学基金青年项目（31900260） |
|
| Transcriptome-based analysis of the effect of the Arabidopsis thaliana tek/ms188 double mutation on pollen wall development |
|
SU Wenxin, LI Yingyin, CHEN Yijia, LIU Yaqi, XU Xiaofeng
|
|
College of Life Sciences, Shanghai Normal University, Shanghai 200234, China
|
| Abstract: |
| Based on the anther transcriptome data, the regulatory patterns of pollen wall-related genes during anther development in Arabidopsis thalianatek/ms188 mutant were analyzed. Compared with the wild-type transcriptome data, a total of 1 588 differentially expressed genes were detected in the tek/ms188 mutant, of which, 211 （13.3%） were up-regulated and 1 377 （86.7%） were down-regulated. GO enrichment analysis enriched a total of 1 139 differential genes, with 176 up-regulated （15.5%） and 963 down-regulated （84.5%）. The differential genes were mainly concentrated in the pollen tube growth and cell wall regulation processes in biological processes （46.6%）. In pollen tube clustering, a large number of down-regulated genes were mainly concentrated in cell wall composition. At the same time, KEGG analysis found that there was a specific expression of UDP-glucose pyrophosphorylase （USP） in the metabolic pathways of pentose and gluconate. The preliminary results of this study suggest that genes related to pollen tube wall development are also involved in pollen wall development. By analyzing the effect of the tek/ms188 double mutant on male fertility in Arabidopsis thaliana, we will analyze the developmental process of male reproduction in plants in depth, and provide some theoretical support for the research process of the third generation hybrid rice. |
| Key words: transcriptome analysis pollen tube development pollen wall development TEK MS188 |
