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| 药用植物丹参中丹参酮IIA的提取分离工艺研究 |
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张毅1, 邓昌平1, 时敏1, 付蓉1, 王瑶2, 曹建国1, 开国银1,2
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1.上海师范大学 生命科学学院 植物种质资源开发协同创新中心, 上海 200234;2.浙江中医药大学 药学院, 浙江 杭州 311402
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| 摘要: |
| 石油醚、乙酸乙酯作为萃取剂，利用聚酰胺凝胶柱分离，乙醇/水梯度洗脱以分离丹参（Salvia miltiorrhiza），得到丹参酮ⅡA.所得到的产物经过核磁共振谱（1H NMR、13C NMR）确证了结构，并经薄层色谱（TLC）、高效液相色谱（HPLC）分析，显示其纯度达到93%以上.本研究提供了一种高效的丹参酮ⅡA的提取分离方法，具有提取率高、成本低、安全可靠等优点，也为其他药用植物有效成分的分离提供参考. |
| 关键词: 丹参(Salvia miltiorrhiza) 丹参酮IIA 纯化 聚酰胺 柱层析 |
| DOI：10.3969/J.ISSN.1000-5137.2018.06.016 |
| 分类号:R932 |
| 基金项目:国家自然科学基金（81522049，31270007，31571735，30900110）；上海市科学技术委员会项目（17JC1404300，15430502700）；上海市曙光学者计划（16SG38）；浙江省高校高水平创新团队资助；上海市协同创新中心项目（ZF1205）；上海植物种质资源工程技术研究中心项目（17DZ2252700） |
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| Purification of tanshinone IIA from medicinal plant Salvia miltiorrhiza |
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ZHANG Yi1, DENG Changping1, SHI Min1, FU Rong1, WANG Yao2, CAO Jianguo1, KAI Guoyin1,2
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1.Development Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China;2.College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 311402, Zhejiang, China
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| Abstract: |
| In this study,petroleum ether and ethyl acetate were used as extraction agents,and polyamide column chromatography was used to separate tanshinone ⅡA using ethanol/water as eluting solvents.TLC,1H NMR,13C NMR,HPLC analysis revealed that the purity of the extracted tanshinone ⅡA was over 93%.Above all,this study provides a highly efficient extraction and separation method for tanshinone ⅡA which displayed a series of advantages including higher yield,lower cost,higher safety and reliability,etc.And it also provides a new approach for purification of other active ingredients from medicinal plants. |
| Key words: Salvia miltiorrhiza tanshinone IIA purification polyamide column chromatography |
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