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叶绿体巯基亚硝基化修饰蛋白质组学研究进展
董美辰1, 喻娟娟1,2, 秦智1, 戴绍军1
1.上海师范大学 生命科学学院 植物种质资源开发协同创新中心, 上海 200234;2.东北林业大学 盐碱地生物资源环境研究中心, 黑龙江 哈尔滨 150040
摘要:
蛋白质S-亚硝基化是NO与蛋白质的半胱氨酸残基共价连接形成S-亚硝基硫醇的过程.叶绿体内快速变化的氧化还原环境容易导致蛋白质S-亚硝基化修饰发生.综述了植物逆境应答过程中叶绿体蛋白质S-亚硝基化参与调节的光合电子传递、卡尔文循环、抗氧化系统、蛋白质合成、蛋白质加工与周转、Ca2+介导的信号转导、氮同化、硫同化,以及四吡咯化合物合成等途径,为全面理解植物逆境应答过程的NO调控网络机制提供了线索.
关键词:  植物  叶绿体  S-亚硝基化修饰  蛋白质组学
DOI:10.3969/J.ISSN.1000-5137.2018.06.001
分类号:Q946.1
基金项目:上海市科学技术委员会科研计划项目(17391900600);上海市协同创新中心项目(ZF1205);上海植物种质资源工程技术研究中心项目(17DZ2252700)
Advances on plant chloroplast S-nitrosylation proteomics
DONG Meichen1, YU Juanjuan1,2, QIN Zhi1, DAI Shaojun1
1.Development Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China;2.Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Harbin 150040, Heilongjiang, China
Abstract:
Protein S-nitrosylation is a NO signal transduction pathway,in which NO is covalently linked to a protein cysteine residue to form an S-nitrosothiol.The rapidly changing redox environment in the chloroplasts easily leads to protein S-nitrosylation.In this study,we reviewed that the photosynthetic electron transport,Calvin cycle,antioxidant system,protein synthesis,protein processing and turnover,Ca2+-mediated signaling,nitrogen and sulfur assimilation,as well as tetrapyrrole biosynthesis which were modulated by protein S-nitrosylation.These studies provide important clues for a comprehensive understanding of the NO signaling regulatory network in plants.
Key words:  plant  chloroplast  S-nitrosylation  proteomics