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流式细胞仪检测技术在食源性致病菌检测中的应用
梅仕良1, 姜凯1, 张娜娜1, 郑亦舟1, 王杰1, 周昱曦2, 赵渝1
1.上海师范大学 生命与环境科学学院 植物种质资源开发协同创新中心, 上海 200234;2.贝克曼库尔特商贸(中国)有限公司, 上海 200122
摘要:
流式细胞术是一种灵敏度高,耗时短,程序简便的定量细胞分析分选方法,广泛应用于微生物检测、环境监测、临床医学等领域,尤其在微生物检测中表现突出.流式细胞术正逐步应用于食源性致病菌的检测,研究并介绍了流式细胞术在几种食源性致病菌检测中的应用,该方法具有广阔的发展前景.
关键词:  流式细胞术  微生物  食源性致病菌  快速检测
DOI:10.3969/J.ISSN.1000-5137.2017.05.021
分类号:Q93-338
基金项目:上海市2017年度"科技创新行动计划"(1714220300);上海植物种质资源工程技术研究中心项目(17DZ2252700)
Application of Flow Cytometry technique in detection of foodborne pathogens
Mei Shiliang1, Jiang Kai1, Zhang Nana1, Zheng Yizhou1, Wang Jie1, Zhou Yuxi2, Zhao Yu1
1.Development Center of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;2.Beckman Coulter Trading(China) Limited, Shanghai 200122, China
Abstract:
Flow Cytometry technique is a quantitative cell analysis method with characteristics such as high sensitivity,time-saving,simple procedure,which is widely applied in microbial detection,environmental monitoring,clinical medicine and other fields,especially in microbial detection. Flow Cytometry is being gradually applied in the detection of foodborne pathogens. This study introduced the application situation of Flow Cytometry technique on several foodborne pathogenic bacteria. It shows the technology has many advantages and a vast development future.
Key words:  Flow Cytometry  microorganism  foodborne pathogens  quick analysis