| 摘要: |
| 由于传统的pH计不能很好地应用在微环境(如亚细胞器)和复杂生物体系中的pH值动态和精确定量检测,因此迫切需要研究可监测复杂多变生物体系中pH值变化且具有高灵敏度的pH值响应分子探针.就近年来用于生物体系(主要是活细胞内)pH值检测的小分子荧光探针作简要综述,同时对目前基于有机小分子构建的荧光探针用于生物体系pH值检测研究及其未来的发展趋势进行展望. |
| 关键词: pH值 比色/荧光 检测 荧光探针 |
| DOI： |
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| 基金项目:国家自然科学基金面上项目(21373138,21672150);国家自然科学基金青年科学基金(21302125);教育部博士点新教师基金(20133127120005);上海市晨光计划(14CG42) |
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| Progress of fluorescent probes based on small organic molecules for pH value detection in biological systems |
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WU Luling, HUANG Chusen, JIA Nengqin
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Key Laboratory of Resource Chemistry,Ministry of Education,College of Life and Environmental Sciences,Shanghai Normal University
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| Abstract: |
| As traditional pH meters cannot work well for the real-time determination and quantitative detection of pH value changes in microenvironments (such as subcellular organelles) and complex biological systems,molecular pH-sensitive probes for monitoring pH changes in complicated and changeable biological systems are urgently needed.This review focuses on the current progress in small molecular fluorescence probes for the detection of pH value changes in biological systems (mainly in monitoring the pH fluctuation in the live cell).Future development of fluorescent probes for pH value detection in the biological systems was also discussed. |
| Key words: pH value colorimetric/fluorescence detection fluorescent probe |
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