| 摘要: |
| 小链霉菌(Streptomyces parvus)HCCB10043的主要代谢产物为脂肽类化合物A21978C,其基因组序列中包括了非核糖体肽合成酶(NRPS non ribosomal peptide synthetase)、聚酮合酶(PKS polyketide synthases)以及 NRPS -PKS混合的多酶体系基因簇,它们的共同特点是在代谢产物生物合成簇中连接有一个硫酯酶域,即TE(thioesterase)domain.硫酯酶可以使已经合成的化合物链的合成过程终止,并且具有水解释放成熟脂肽以及环化线性脂肽链的功能.通过对联二吡啶类代谢产物合成簇中TE基因的敲除构得工程菌株,工程菌发酵结果表明联二吡啶类代谢产物产量减少. |
| 关键词: 基因敲除 工程菌株 硫酯酶 代谢产物 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金(81102354) |
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| Construction of an engineering strain which knocked out the gene of thioesterase for Streptomyces parvus HCCB10043 and the reach of metabolites |
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YIN Fang1, RAO Min1, WEI Wei1, CHEN Lingling1, CHEN Daijie2
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1.Shanghai Laiyi biopharmaceutical R&D center Biological Laboratory Ⅱ;2.College of pharmacy,Shanghai jiaotong university
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| Abstract: |
| The major metabolites of Streptomyces parvus HCCB10043 is lipopeptide compounds A21978C,its genome sequence includes the non ribosomal peptide synthetase(NRPS),polyketide synthases(PKS) and hybrid NRPS-PKS multi-enzyme system gene clusters,they do have a their common feature in the metabolite biosynthetic cluster,which is called TE domain as well.Thioesterase can synthesized the synthesis of compounds of the chain termination,and with functions to release mature lipopeptide hydrolysis and cyclized peptide chain aliphatic linear.This study,we knockout the TE domain of a gene cluster,which guide the biosynthesis of bipyridine,to obtain engineered bacteria.The fermentation results demonstrates reduced yields for metabolites 2,2′-Bipyridine (2,2′-BP). |
| Key words: gene knockout engineering strain thioesterase metabolites |
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