| 摘要: |
| 在临床实践中，碱性磷酸酶（ALP）是最常检测的酶之一，已被广泛用作肝病、骨病、乳腺癌、前列腺癌以及糖尿病等多种疾病的重要检测指标.通过自组装方法在氧化铟锡（ITO）导电玻璃上制备金纳米线（AuNWs），利用4-巯基苯甲酸（4-MBA）修饰AuNWs，对其进行改性，与铜离子（Cu2+）螯合形成Cu2+-AuNWs/ITO电极.在ALP活性检测中，电解液中的焦磷酸根阴离子（PPi）与电极表面Cu2+结合，湮灭了Cu2+的电化学信号.ALP的加入使底物PPi水解并释放出Cu2+，电化学信号增加.构建的Cu2+-AuNWs/ITO电极对ALP活性检测范围为60~180 U·L-1，且抗干扰性能良好. |
| 关键词: 碱性磷酸酶(ALP) 金纳米线(AuNWs) 无标记检测 电化学传感器 |
| DOI：10.3969/J.ISSN.1000-5137.2022.05.003 |
| 分类号:O647.3 |
| 基金项目:国家自然科学基金（21475088） |
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| Indirect detection of alkaline phosphatase activity by electrochemical sensor based on gold nanowires |
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YU Tingting, WANG Min, WEN Ying, YANG Haifeng
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College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China
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| Abstract: |
| In the clinical practice, alkaline phosphatase (ALP) is one of the most frequently detected enzymes. It has been used as an important indicator of liver disease, bone disease, breast cancer, prostate cancer, diabetes, and other diseases. In this report, gold nanowires(AuNWs) were firstly prepared on indium tin oxide(ITO) conductive glass by self-assembly method. Then, AuNWs were modified by 4-mercaptobenzoic acid (4-MBA) and chelated with copper ions(Cu2+) to form the Cu2+-AuNWs/ITO electrode. In the detection of ALP activity, pyrophosphate anion(PPi) in the electrolyte combines with copper ions on the electrode surface to annihilate the electrochemical signal of copper ions. However, the addition of ALP hydrolyzes the substrate PPi and releases copper ions, which increases the electrochemical signal. The as-constructed Cu2+-AuNWs/ITO electrode can detect ALP activity in the range of 60~180 U·L-1 with the high anti-interference performance. |
| Key words: alkaline phosphatase(ALP) gold nanowires(AuNWs) unmarked detection electrochemical sensor |
