| 摘要: |
| 在聚甲基丙烯酸甲酯-微针（PMMA-MNs）阵列上修饰聚多巴胺（PDA）层，通过离心技术将金纳米粒子（Au NPs）组装在MNs表面，构建纳米金@聚多巴胺@聚甲基丙烯酸甲酯微针阵列（Au@PDA@MNs）.Au@PDA@MNs有强表面增强拉曼散射（SERS）效应，以罗丹明6G（R6G）作为拉曼探针，在10-4~10-8 mol·L-1的范围内有良好的线性响应，检测限（LOD）可达3.1×10-10 mol·L-1.为了检测过氧化氢（H2O2），4-巯基苯硼酸（4-MPBA）被修饰在Au@PDA@MNs上以构建功能化的MNs.利用4-MPBA分子氧化生成4-羟基硫代苯酚（4-HTP）和MNs的透光性，基于1 000 cm-1和1 068 cm-1处拉曼强度比（I1 000/I1 068）的变化，完成了H2O2的拉曼定量检测，I1 000/I1 068的变化与H2O2浓度成定量关系.线性响应范围为10~2 000 μmol·L-1，LOD为0.93 μmol·L-1.MNs和SERS技术的结合为确定具有短半衰期和弱拉曼信号的疾病相关生物标志物开辟了一条新途径. |
| 关键词: 微针(MNs) 原位 表面增强拉曼 过氧化氢(H2O2) |
| DOI：10.3969/J.ISSN.1000-5137.2022.05.002 |
| 分类号:O657.1 |
| 基金项目:国家自然科学基金（21475088） |
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| In situ detection of H2O2 based on transparent microneedles Raman |
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PENG Cheng, WANG Feng, YANG Haifeng
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College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China
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| Abstract: |
| Polydopamine(PDA) was modified on the surface of polymethyl methacrylate microneedles(PMMA-MNs) array, and then gold nanoparticles(Au NPs) were assembled on the surface of the microneedles by using centrifugation, designated as Au@PDA@MNs. The as-prepared Au@PDA@MNs have surface enhanced Raman scattering(SERS) effect. In case of Rhodamine 6G(R6G) as the Raman probe, a good linear response in the range of 10-4-10-8 mol·L-1 was achieved with the limit of detection(LOD) of 3.1×10-10 mol·L-1. For detection of hydrogen peroxide(H2O2), 4-mercaptophenylboronic acid(4-MPBA) was modified on Au@PDA@MNs to construct functionalized MNs.Based on the change of Raman intensity ratio(I1 000/I1 068) at 1 000 cm-1 and 1 068 cm-1, Raman quantitative detection of H2O2 was completed by utilizing the oxidation of 4-MPBA molecule to generate 4-hydroxythiophenol(4-HTP) and the light transmittance of MNs.The change of I1 000/I1 068 has a quantitative relationship with the concentration of H2O2. The linear response range of 10~2 000 μmol·L-1 and the LOD of 0.93 μmol·L-1 were reached. The combination of MNs and SERS techniques paves a new route to determine disease-related biomarkers with short half-life and weak Raman signals. |
| Key words: microneedles(MNs) in situ surface enhanced Raman scattering hydrogen peroxide(H2O2) |
