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| 辐射诱变小兰屿蝴蝶兰叶片生长与表型差异品种间ISSR分析 |
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李威1,2, 宋子涵1,2, 何国仁1,2, 陈佳瀛1,2, 明凤1,2
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1.上海师范大学 生命科学学院, 上海 200234;2.上海师范大学 植物种质资源开发协同创新中心, 上海 200234
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| 摘要: |
| 使用60Co-γ射线对小兰屿蝴蝶兰进行辐射处理,分别对单唇瓣、三唇瓣品种采用15 Gy和20 Gy剂量的处理,采用简单重复序列区间扩增多态（ISSR）分子标记技术对经辐射处理的小兰屿蝴蝶兰材料进行遗传多样性和亲缘关系分析. 筛选出8条引物,共扩增出89条清晰的谱带,其中72条表现出多态性,多态比例为80.9%. 单唇瓣品种之间遗传相似范围在0.54~0.97,三唇瓣品种之间遗传相似范围在0.54~0.91,说明辐射突变品种之间有丰富的遗传多态性. 单唇瓣小兰屿蝴蝶兰经过辐射后,在遗传距离L=0.65处可分为4组,三唇瓣小兰屿蝴蝶兰经过辐射后,在遗传距离L=0.72处可分为7组. 观察生长表型发现：经辐射处理的小兰屿蝴蝶兰出现生长快速表型,部分经辐射处理的单唇瓣株系生长率比对照组更高,而经辐射处理的三唇瓣株系生长率普遍比对照组高. 非加权组平均法（UPGMA）聚类分析表明：小兰屿蝴蝶兰经过辐射诱变后有不同程度的突变,突变程度最大的品种在遗传距离图谱上自成一支. 以上结果为培育优质蝴蝶兰品种奠定了材料基础. |
| 关键词: 60Co-γ射线 物理诱变 简单重复序列区间扩增多态（ISSR） 分子育种 小兰屿蝴蝶兰 |
| DOI：10.3969/J.ISSN.1000-5137.2024.03.011 |
| 分类号:Q947.9 |
| 基金项目:国家重点研发计划（2018YFD1000404）；上海植物种质资源工程技术研究中心项目（17DZ2252700）；上海市地方院校能力建设专项（20070502500） |
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| ISSR analysis of different varieties of Phalaenopsis equestris leaf growth and phenotypes induced by radiation |
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LI Wei1,2, SONG Zihan1,2, HE Guoren1,2, CHEN Jiaying1,2, MING Feng1,2
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1.School of Life Sciences, Shanghai Normal University, Shanghai 200234, China;2.Collaborative Innovation Center for Plant Germplasm Resources Development, Shanghai Normal University, Shanghai 200234, China
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| Abstract: |
| The Phalaenopsis equestris was irradiated with 60Co-γ rays, and its provariety and trilabial mutant varieties were treated with 15 Gy and 20 Gy doses respectively. Then the inter-simple sequence repeat （ISSR） molecular marker technology was used to irradiate the P. equestris plants. The materials were analyzed for their genetic diversity and genetic relationship. Eight primers were screened to amplify a total of 89 clear bands, of which 72 showed polymorphism, with a polymorphism ratio of 80.9%. The range of genetic similarity between provariety of P. equestris is 0.54-0.97, and the range of genetic similarity between the trilabial mutant varieties of P. equestris is 0.54-0.91, indicating that there are rich genetic polymorphisms among radiation experimental mutant varieties. P. equestris can be divided into four groups at genetic distance L=0.65 after radiation, and trilabial mutant varietiescan be divided into seven groups at genetic distance L=0.72 after radiation. Observing the growth phenotype, it was found that the radiation-treated P. equestris showed a fast-growing phenotype, and the growth rate of some P. equestris lines treated with radiation was higher than that of the control group； while the trilabial mutant varieties lines treated with radiation generally had a higher growth rate than the control group. UPGMA （unweighted pair-group method with arithmetic means） cluster analysis showed that P. equestris had different degrees of mutations after radiation mutagenesis, and the varieties with the largest mutations formed their own branches on the genetic distance map. The results above laid the material foundation for the cultivation of Phalaenopsis. |
| Key words: 60Co-γ rays physical mutagenesis inter-simple sequence repeat （ISSR） molecular breeding Phalaenopsis equestris |
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