| 摘要: |
| 生物膜是由细菌及其分泌的胞外聚合物组成的微生物簇. 目前,生物膜培养和研究多采用静态培养平台（如培养皿和孔板）,这种方式缺乏精准模拟理化微环境、高通量以及原位分析细菌行为的能力. 近年来,微流控技术作为一种在微米尺度处理和操纵流体的新技术,在生物膜研究中的应用不断增加,且取得了一系列突破性进展. 文章系统综述了最新的基于微流控技术的生物膜研究成果,总结并展望了微流控技术辅助生物膜研究的优缺点及未来方向,以期为全面了解和拓展微流控技术在生物膜研究中的应用提供帮助. |
| 关键词: 微流控技术 生物膜 高通量 可视化分析 单细胞水平 |
| DOI：10.3969/J.ISSN.1000-5137.2024.01.015 |
| 分类号:Q 939 |
| 基金项目:国家自然科学基金（31971310, 32371439） |
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| Application of microfluidic technology in biofilm research |
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WANG Shanshan, YIN Min, ZHANG Fengjuan, YU Ziying, CHEN Nan, LYU Min
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College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China
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| Abstract: |
| Biofilm is a microbial community composed of bacteria and their secreted extracellular polymers. Currently, biofilm culture and research mainly use static culture platforms（such as Petri dishes and well-plate）, which lack the ability to accurately simulate physicochemical microenvironments and perform high-throughput in situ analysis of bacterial behavior. Microfluidics, a new technique for processing or manipulating fluids at the micron scale, has been used in biofilm research in recent years and several breakthroughs have been achieved. This paper systematically reviews recent research on the application of microfluidic technology to biofilms and summarizes the advantages, disadvantages, and future directions of microfluidic technology-assisted biofilm research to provide assistance for a comprehensive understanding and extension of the application of microfluidic technology in biofilm research. |
| Key words: microfluidic technology biofilm high-throughput visual analysis single cell level |
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