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| 高效液相色谱测定乳液和化妆水中芍药苷和葛根素的含量 |
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胡小荣, 王艺泽, 楼芝英, 胡岗, 周国光
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上海师范大学 化学与材料学院, 上海 200234
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| 摘要: |
| 建立了高效液相色谱（HPLC）检测乳液和化妆水中芍药苷和葛根素的方法.样品经过甲醇超声提取，以质量分数为0.1%磷酸水溶液-乙腈洗脱，经C18反相色谱柱分离，二极管阵列检测器（DAD）检测，计算出2种草药成分含量.在最优化条件下，该方法对芍药苷和葛根素的相应线性范围在0.2~10.0μg·mL-1，在232 nm和250 nm处紫外吸收强度具有良好的线性关系，相关系数（R2）大于0.999.该方法的回收率在90.4%~103.9%之间，相对标准偏差（RSD）在1.53%~2.37%之间，芍药苷和葛根素在乳液和化妆水中的方法检出限分别是2 mg·kg-1和8 mg·kg-1.该方法适合快速、高效、准确测定乳液和化妆水中芍药苷和葛根素的含量. |
| 关键词: 高效液相色谱(HPLC) 芍药苷 葛根素 乳液 化妆水 |
| DOI：10.3969/J.ISSN.1000-5137.2021.05.013 |
| 分类号:O657.1 |
| 基金项目:上海市科技专项（19142200200） |
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| Determination of paeoniflorin and puerarin in emulsion and cosmetic water with HPLC |
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HU Xiaorong, WANG Yize, LOU Zhiying, HU Gang, ZHOU Guoguang
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College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China
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| Abstract: |
| A high performance liquid chromatography (HPLC) method for determination of paeoniflorin and puerarin in emulsion and cosmetic water was established. The samples were extracted with methanol by ultrasonication, and separated with acetonitrile-0.1% (mass fraction) phosphoric acid aqueous solution by gradient elution using C18 RP column and then inspected by diode array detector (DAD) detector. Under the optimum conditions,The linear relationship of the concentrations of paeoniflorin and puerarin and the intensity of UV absorptions at 232 nm and 250 nm was good in a range of 0.2-10.0 μg·mL-1,and the squared correlation coefficient (R2) was greater than 0.999. The recovery rate of two herbs was between 90.4% and 103.9%, and the relative standard deviation (RSD) was between 1.53% and 2.37%. The detection limit was between 2 mg·kg-1 and 8 mg·kg-1, meeting the requirements of the national standard for the detection limit. The developed method is suitable for the rapid, efficient, and accurate determination of paeoniflorin and puerarin in emulsion and cosmetic water. |
| Key words: high performance liquid chromatography(HPLC) paeoniflorin puerarin emulsion cosmetic water |
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