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| 利用CRISPR/Cas9技术快速创制香型“秀水134”水稻 |
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赵国超1,2, 朱小方1,2, 王彤1,2, 李建粤1,2
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1.上海师范大学 生命科学学院, 上海 200234;2.上海师范大学 上海植物种质资源工程技术研究中心, 上海 200234
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| 摘要: |
| 以目前上海市主栽的高产常规水稻"秀水134"为材料，利用CRISPR/Cas9技术成功敲除甜菜碱醛脱氢酶2基因，获得了两种类型纯合突变体植株.采用表达载体特异性结合的引物检测T1代转基因植株，成功获得6株不携带载体骨架的转基因植株.定量PCR分析显示，突变体植株甜菜碱醛脱氢酶2基因表达量极显著低于野生型对照（p<0.01），但突变体植株成熟种子香味物质2-乙酰-1-吡咯啉（2AP）含量极显著高于野生型对照（p<0.01）.比较野生型对照与突变体植株的主要农艺性状和产量性状，两者间都没有显著差异（p>0.05）.本研究可为加快高产香型水稻在上海及周边地区的推广应用，以及为今后利用CRISPR/Cas9技术快速培育其他高产香型水稻新品种研究奠定基础. |
| 关键词: 水稻 甜菜碱醛脱氢酶2基因 2-乙酰-1-吡咯啉(2AP) CRISPR/Cas9基因编辑技术 “秀水134” |
| DOI：10.3969/J.ISSN.1000-5137.2019.05.015 |
| 分类号:S511 |
| 基金项目:上海市科委现代农业科技创新项目（16391900800）；上海植物种质资源工程技术研究中心项目（17DZ2252700） |
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| Breeding fragrant rice “Xiushui 134” using CRISPR/Cas9 mediated gene editing technology |
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ZHAO Guochao1,2, ZHU Xiaofang1,2, WANG Tong1,2, LI Jianyue1,2
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1.College of Life Sciences, Shanghai Normal University, Shanghai 200234, China;2.Shanghai Engineering Research Center of Plant Germplasm Resources, Shanghai Normal University, Shanghai 200234, China
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| Abstract: |
| In the study,fragrance rice mutant lines were developed with CRISPR/CAS9 by editing fragrance gene Badh2 in "Xiushui 134",a major elite rice cultivar in Shanghai region.Six mutant lines without the vector backbone were successfully obtained by PCR detection.Quantitative PCR analysis showed that the expression of Badh2 in mutant plants was significantly lower than that in wild type (p<0.01),the content of 2AP in mature seed of mutant plants was significantly higher than that in wild type (p<0.01),but there were no significant differences on main agronomic traits between the mutant plants and wild type (p>0.05).This research can accelerate the application of high-yield fragrant rice in Shanghai areas,and lay the foundation for the future study on the rapid cultivation of other high-yield fragrant rice varieties using CRISPR/Cas9 technology. |
| Key words: rice betaine aldehyde dehydrogenase 2 gene 2AP CRISPR/Cas9 gene editing technology “Xiushui 134” |
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