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| 利用分子标记辅助选育香软型保持系“SH101B”、不育系“SH101A”及配组分析 |
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王彤1,2, 曾文秀1,2, 王冬翼3, 赵国超1,2, 李建粤1,2
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1.上海师范大学 生命科学学院, 上海 200234;2.上海师范大学 上海植物种质资源工程技术研究中心, 上海 200234;3.上海市浦东新区农业技术推广中心, 上海 201201
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| 摘要: |
| 为了给三系杂交稻新品种选育提供优良亲本，以"嘉花1号"水稻为转育亲本，与香型软米水稻杂交，再与"嘉花1号"水稻多代回交及自交，结合分子标记辅助选育香软型水稻新品系"SH101B"."嘉花1号"与"SH101B"水稻主要农艺性状和产量性状差异都不显著（p>0.05）."SH101B"稻米直链淀粉含量（质量分数）为10.0%，明显低于"嘉花1号"水稻（15.2%）.又将"SH101B"与三系不育系水稻杂交和回交，转育获得香软型三系不育系"SH101A"，再与籼型恢复系"T201"进行配组，并对后代农艺性状、产量和稻米品质进行分析.结果显示，"SH101A×T201"组合稻米直链淀粉含量为12.8%，每亩产量达到848.1 kg（12 726.4 kg·hm-2）. |
| 关键词: 香软水稻 分子标记辅助选育 “SH101B” “SH101A” 杂交组合 |
| DOI：10.3969/J.ISSN.1000-5137.2019.05.012 |
| 分类号:S511.037 |
| 基金项目:上海市农委种业项目（沪农科种字（2016）第1-7号）；上海植物种质资源工程技术研究中心项目（17DZ2252700） |
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| Development of selectively aromatic soft maintainer line “SH101B” and sterile line “SH101A” by molecular marker-assisted selection and analysis of hybrid combination |
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WANG Tong1,2, ZENG Wenxiu1,2, WANG Dongyi3, ZHAO Guochao1,2, LI Jianyue1,2
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1.College of Life Sciences, Shanghai Normal University, Shanghai 200234, China;2.Shanghai Engineering Research Center of Plant Germplasm Resources, Shanghai Normal University, Shanghai 200234, China;3.Agricultural Technology Extension and Service Center of Pudong New Area, Shanghai 201201, China
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| Abstract: |
| In order to provide excellent parents for breeding new cytoplasmic male sterility(CMS) line rice varieties,we developed a new rice strain,"SH101B",by molecular marker-assisted gene pyramiding,using hybridizing between non-fragrant and non-soft rice "Jiahua No.1" with a fragrant soft rice,and backcrossing with "Jiahua No.1" rice for multi-generation,and then selfcrossing.There were no significant differences between "Jiahua No.1" and "SH101B" in main agronomic and yield traits (p>0.05).The amylose content (mass fraction) of "SH101B" rice was 10.0%,which was significantly lower than that of "Jiahua No.1" rice (15.2%).In this study,"SH101B" was crossed and backcrossed with three-line sterile line rice to obtain fragrant and soft three-line sterile line "SH101A",which was then paired with Indica restorer line "T201",and its offspring were analyzed for agronomic traits,yield and rice quality.The results showed that the amylose content of "SH101A×T201" rice was 12.8% and the yield reached 12 726.4 kg·hm-2. |
| Key words: fragrant and soft rice molecular marker assisted breeding “SH101B” “SH101A” hybrid combinations |
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