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扇叶铁线蕨总黄酮和总酚含量及其抗氧化能力分析
张昕, 王梦颖, 李懿宸, 曹建国, 戴锡玲
上海师范大学 生命与环境科学学院 植物种质资源开发协同创新中心, 上海 200234
摘要:
测定了扇叶铁线蕨(Adiantum flabellulatum L.)的总黄酮和总酚含量及其对DPPH (DPPH·)自由基、ABTS自由基(ABTS·)的清除力和Fe的还原力.结果表明:1)扇叶铁线蕨总黄酮质量分数在6%~8%之间,总酚质量分数在7%~14%之间,地下部分总黄酮和总酚含量高于地上部分,不同产地的扇叶铁线蕨总黄酮和总酚含量存在一定差异;2)扇叶铁线蕨提取物具有一定的Fe还原能力,以及清除DPPH自由基和ABTS自由基的能力;且ABTS自由基清除能力和Fe还原能力随总黄酮和总酚浓度的增加而增强.
关键词:  扇叶铁线蕨  总黄酮  总酚  抗氧化能力
DOI:10.3969/J.ISSN.1000-5137.2017.05.011
分类号:R284
基金项目:上海市自然科学基金(15ZR1430500);上海市科学技术委员会课题(14DZ2260400);上海市绿化和市容管理局科学技术项目(G152430);上海植物种质资源工程技术研究中心项目(17DZ2252700)
Total flavonoids and total phenol content and their antioxidant activities of fern Adiantum flabellulatum
Zhang Xin, Wang Mengying, Li Yichen, Cao Jianguo, Dai Xiling
Development Center of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China
Abstract:
The contents of total flavonoids and total phenol of fern Adiantum flabellulatum L. were determined. The DPPH and ABTS free radical scavenging activities and Fe reduction force were determined and analyzed as well. The results showed:1) The total flavonoid content of A. flabellulatum ranged from 6%~8%,while the total phenol contents ranged from 7%~14%. Total flavonoid and total phenol contents in subterranean parts were both higher than those in aerial parts. And total flavonoid and total phenol contents were different for material from different localities;2) The extracts from A. flabellulatum have certain ferric reducing ability of plasma and DPPH and ABTS radicals scavenging activities. And ABTS scavenging activity and ferric reducing ability of plasma both strengthen along with the increasing of concentration of total flavonoids and total phenol respectively.
Key words:  Adiantum flabellulatum L.  total flavonoids  total phenol  antioxidant capacity