| 摘要: |
| 通过缓慢杀线实验、偏好性实验和共培养毒性实验分析了秀丽线虫（Caenorhabditis elegans）对荧光假单胞菌P13（Pseudomonas fluorescens P13）和解淀粉芽孢杆菌S3-1（Bacillus amyloliquefaciens S3-1）传播的可能性.通过显微观察与平板稀释涂布对秀丽线虫细菌携带作用进行了定性、定量分析，并对细菌-线虫-植物三者交互作用进行初步探究.结果发现，P13和S3-1对秀丽线虫的慢性致死率分别为12.12%和3.00%，每10 s身体弯曲次数分别为4.68和4.33.相对于尿嘧啶缺陷型大肠杆菌（OP50），线虫对P13和S3-1选择系数分别为0.13和0.52，P13、S3-1携带菌量分别为（4.02×103±47）和（9.67×102±22） CFU/条.携细菌线虫将细菌定向传播至植物根际，细菌定殖菌量为105 CFU，有效促进植物生长发育. |
| 关键词: 秀丽线虫 根际益生菌 荧光假单胞菌P13 解淀粉芽孢杆菌S3-1 |
| DOI：10.3969/J.ISSN.1000-5137.2017.04.002 |
| 分类号:Q932 |
| 基金项目:上海市2016年度"科技创新行动计划"项目（16391902100） |
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| The effects of Caenorhabditis elegans on plant growth-promoting rhizobacteria and plant growth |
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Liu Xingxing, Yang Wenwu, Chen Jiajia, Xiao Ming
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College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China
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| Abstract: |
| The diversity and ecology functions of soil nematodes have gained comprehensive attentions.In this study,we conducted a series of experiments (the slow killing assay,long time toxicity experiment and preference experiment)to test whether nematodes Caenorhabditis elegans(C.elegans) may contribute to the spread of the P13 and S3-1.Combining microscopic observation with dilution-plate method,we analyzed the bacteria carried by C.elegans.Furthermore,we preliminarily explored the interactions of bacteria,nematodes and plants.Death rates of C.elegans in slow killing assay driven by two strains were 12.12% (P13) and 3.0% (S3-1),separately.The body bending frequencies of C.elegans were 4.68/10s (P13) and 4.33/10s (S3-1),respectively.Choice indexes of P13 and S3-1 were 0.13 and 0.52,separately.The total numbers of bacteria carried by C.elegans were 4.02×103±47 (P13) and 9.67×102±22 (S3-1).C.elegans carried bacteria to the rhizosphere where the bacteria content was as high as 105 CFU per plant root.As a vector of rhizosphere bacteria,C.elegans can carry P13 and S3-1 to plant rhizosphere and effectively promote plant growth and development. |
| Key words: Caenorhabditis elegans plant growth-promoting rhizobacteria Pseudomonas fluorescens P13 Bacillus amyloliquefaciens S3-1 |
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