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单增李斯特菌的检测方法研究进展
李云霞, 陈雯雯, 顾晨荣, 饶名祯, 郭鲁申, 赵渝
上海师范大学
摘要:
单增李斯特菌(Listeria monocytogenes)是全世界普遍公认一种重要的人畜共患食源性致病菌,其广泛存在于各种乳制品、蔬菜、肉类等食品中,对人的健康安全造成了极大的威胁.因此,如何有效地检测出单增李斯特菌的存在是食源性疾病预防与控制的关键环节.介绍了单增李斯特菌的传统检测方法以及各种快速检测方法(免疫学方法、生物传感器、基于噬菌体检测方法和分子生物学方法).
关键词:  单增李斯特菌  食源性致病菌  检测
DOI:
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基金项目:上海市教委重点项目(14ZZ123);上海师范大学一般科研项目(SK200906)
Research advancement of detection methods of Listeria monocytogenes
LI Yunxia, CHEN Wenwen, GU Chenrong, RAO Mingzhen, GUO Lushen, ZHAO Yu
Development Center of Plant Germplasm Resources,College of Life and Environmental Sciences,Shanghai Normal University
Abstract:
Listeria monocytogenes is a food-borne pathogen of both human beings and animals,which has been widely recognized over the world.L.monocytogenes can be found in dairy products,vegetables,meat and other food,so it has caused great threat to human health.How to detect the trace of L.monocytogenes is a key link in the process of food-borne disease prevention and control.A wide variety of culture and alternative methods have been developed in order to detect this pathogen in food.The current articl introduces the traditional detection method and all kinds of rapid methods such as immunoassay,biosensors,Bacteriophage-based detection methods and molecular biological assay.
Key words:  Listeria monocytogenes  food-borne pathogen  detection