| 摘要: |
| 基于ISSR分子标记,对采自浙江磐安大盘山、临安清凉峰、临安昌化、金华北山双龙洞附近、金华北山朝真洞附近和上海奉贤(栽培)的6个鸭儿芹(Cryptotaenia japonica Hassk.)群体的70个样本进行了遗传多样性分析.从9条筛选出的ISSR引物共扩增出149条带,其中136条具有多态性,多态性条带百分率为91.28%.基于ISSR标记计算了6个鸭儿芹群体的遗传多样性指数,发现它们的Nei的基因多样性指数(H)为0.1971,Shannon信息多态性指数(I)为0.3086,Nm指数为0.3343.6个群体遗传分化系数Nm为0.5993,表明59.93%的遗传分化存在于群体间,40.07%的遗传分化存在于群体内,群体群体间的基因流为0.3237,表明供鸭儿芹群体之间基因流较小,遗传漂变已造成鸭儿芹群体之间较强的遗传分化.基于136个位点,构建了反映各样本间遗传亲缘关系的系统聚类图,结果表明,从70个样本中可以区分出5个类群,其中金华北山采的两个群体为一个类群,与地理位置有很强的对应性.因此,在今后的鸭儿芹引种栽培时,应注意从不同产地的鸭儿芹种源中进行引种,以丰富栽培种的遗传多样性. |
| 关键词: 鸭儿芹 ISSR分子标记 聚类分析 遗传多样性 |
| DOI： |
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| 基金项目:上海市高校知识服务平台-上海市植物种质资源中心经费(ZF1205) |
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| Genetic diversity of six populations of Cryptotaenia japonica in Eastern China based on ISSR markers |
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LIU Xiaohui, SUN Bing, LOU Yuxia, GUO Shuiliang
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Development Center of Plant Germplasm Resources,College of Life and Environmental Sciences,Shanghai Normal University
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| Abstract: |
| Genetic diversity of six populations (including 70 individuals) of Cryptotaenia japonica Hassk,collected from Dapanshan of Pan′an,Qinliangfeng of Lin′an,Chanhua of Lin′an,Beishan of Jinhua,Zhejiang,and Fengxian of Shanghai (cultivated population) were analyzed based on their ISSR markers.A total of 149 bands were amplified from nine selected primers,of which 136 were polymorphic,their percentage of polymorphic bands being 91.28%.Based on the ISSR markers of six C. japonica populations,their Nei′s genetic diversity index,Shannon information index,Nm index was 0.1971,0.3086,and 0.3237,respectively.Their genetic differentiation coefficient (GST) was 0.5993,indicating that 59.93% genetic differentiation exists among populations,and 40.07% genetic differentiation exists within populations.Their gene flow was 0.3343,indicating a low gene flow existed among different populations of C. japonica in the studied areas,namely,a genetic drift has caused a strong genetic differentiation of C. japonica among populations.Based on 136 locus information,a clustering dendrogram was constructed for the 70 individuals to reveal their genetic relationships.Based on the clustering analysis,the individuals could be divided into five groups,among them two populations collected from Jinhuashan were clustered into one group.The genetic differentiation among the five groups has a strong relationship with their geographical localities.Therefore in the future,it should be pay attention that introduce and cultivate Cryptotaonia japonica from different places of origin to enrich the genetic diversity of cultivated species. |
| Key words: Cryptotaenia japonica ISSR molecular marker cluster analysis molecular marker |
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