| 摘要: |
| 碳点（CDs）以其良好的光电性能和生物相容性在电化学发光（ECL）分析领域受到了极大关注,但纯CDs的发光效率较低,需掺杂其他元素来改善其性能. 本文制备了一种氮铈双掺杂的碳点（N-Ce-CDs）,与非掺杂和单元素掺杂CDs相比,其ECL性能得到了明显改善. 将其与金纳米粒子（Au NPs）同时负载于沸石咪唑酯骨架（ZIF-L）表面,得到一种对谷胱甘肽（GSH）具有灵敏响应的ECL探针（N-Ce-CDs,Au@ZIF-L）. 实验中,首先在电极表面引入对GSH具有特异性识别的分子印迹聚合物（MIP）,选择性吸附GSH,再与探针作用. 通过GSH的巯基（-SH）与探针中Au相互作用,将探针带入电极表面,产生ECL信号,实现对GSH的选择性灵敏检测. 在优化的条件下,GSH的物质的量浓度在1.0×10-8~1.0×10-4 mol·L-1范围内与ECL信号呈现良好的线性关系,检出限（LOD）为1.87 nmol·L-1. 对人体血清样品中GSH进行检测,回收率在96.3%~104.8%之间. |
| 关键词: 碳点（CDs） 金纳米粒子（Au NPs） 电化学发光（ECL） 分子印迹聚合物（MIP） 谷胱甘肽（GSH） |
| DOI：10.20192/j.cnki.JSHNU(NS).2025.01.002 |
| 分类号:O657.1 |
| 基金项目:上海师范大学“仪器分析”课程教学团队建设项目（304-AC9103-21-368011523） |
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| The electrochemiluminescence system based on N-Ce-CDs, Au@ZIF-L for glutathione detection |
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TANG Chunyuan1, XIANG Li2, YANG Minli1
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1.College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China;2.Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China
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| Abstract: |
| Carbon dots（CDs） have attracted attention in the field of electrochemiluminescence （ECL） analysis due to their good photoelectric properties and biocompatibility. However, the luminescence efficiency of pure carbon dots is low, so it is necessary to dop other elements to improve the performance. In this paper, a kind of nitrogen-cerium double doped carbon dots （N-Ce-CDs） was prepared. Compared with the undoped and single-element doped CDs, the ECL performance of N-Ce-CDs was significantly improved. It was simultaneously loaded on the surface of zeolite imidazolide skeleton （ZIF-L） with gold nanoparticles（Au NPs）, and an ECL probe （N-Ce-CDs,Au@ZIF-L） with sensitive response to glutathione （GSH） was obtained. In the experiment, molecularly imprinted polymer（MIP） with specific recognition of GSH was initially introduced onto the electrode surface for the selective adsorption of GSH, then reacted with the probe. Through the interaction between the thiol group （-SH） of GSH and the Au in the probe, the probe was brought onto the electrode surface to generate an ECL signal, achieving selective and sensitive detection of GSH. Under the optimized conditions, the mole concentration of GSH showed a good linear relationship with ECL signal in the range of 1.0×10-8-1.0×10-4 mol·L-1, with a limit of detection（LOD） of 1.87 nmol·L-1. The recovery of GSH in human serum samples was 96.3%-104.8%. |
| Key words: carbon dots（CDs） gold nanoparticles（Au NPs） electrochemiluminescence（ECL） molecularly imprinted polymer（MIP） glutathione（GSH） |
