| 摘要: |
| 以现有菠菜基因组信息为基础，通过生物信息学方法筛选鉴定16个菠菜SoSWEET蛋白家族成员，命名为SoSWEET1~SoSWEET16.氨基酸残基数量在648~1 140之间，分子质量在54 070.32~95 868.64 u之间，理论等电点（pI）在5.06~5.19之间.亚细胞定位预测有6个SoSWEET蛋白定位于细胞膜，5个SoSWEET蛋白定位于内质网，5个SoSWEET蛋白定位于细胞膜、内质网.系统进化分析将菠菜SoSWEET蛋白家族分成4个亚族，在此基础上对基因结构、保守基序、顺式作用调控元件等进行分析.共鉴定了10个高度保守基序，其中所有菠菜SoSWEET蛋白都包含基序1，2和4，是构成菠菜SoSWEET蛋白中最高度保守的部分.所有菠菜SoSWEET蛋白家族成员都含MtN3_slv和PQ-loop superfamily结构域.大多数SoSWEET蛋白家族的基因含有5个内含子.顺式作用元件预测结果表明，菠菜SoSWEET基因启动子上包含光响应、生长发育、植物激素响应和逆境胁迫响应等顺式作用元件.组织表达分析表明，所有SoSWEET基因在根、茎、叶和叶柄中都有表达，霜霉病胁迫处理后16个基因表现出不同响应变化.本研究为后续深入研究菠菜SoSWEET蛋白家族成员的功能提供了重要参考. |
| 关键词: 菠菜 SoSWEET蛋白家族 霜霉病 生物信息学分析 |
| DOI：10.3969/J.ISSN.1000-5137.2022.06.003 |
| 分类号:S636.1 |
| 基金项目:上海市科技创新行动计划农业科技领域项目（19391900700）；上海市科技兴农推广项目（沪农科推字（2021）第1-14号） |
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| Identification and expression analysis of SoSWEET protein family genes in spinach |
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YANG Jiao, ZHANG Lijie, WANG Xuemei, WENG Li, LIU Zihan, CAI Xiaofeng
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College of Life Sciences, Shanghai Normal University, Shanghai 200234, China
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| Abstract: |
| Based on the existing spinach genome information, 16 members of spinach SoSWEET protein family were screened and identified by bioinformatics methods. They were named SoSWEET1-SoSWEET16 according to their positions on chromosomes. The number of amino acids ranges from 648 to 1 140, the molecular weight ranges from 54 070.32 to 95 868.64 u, and the isoelectric point (pI) ranges from 5.06 to 5.19. The results of subcellular localization prediction showed that six SoSWEET proteins are located in the membrane, and five SoSWEET proteins are located in the endoplasmic reticulum, and membrane and endoplasmic reticulum, respectively. The phylogenetic tree analysis divided SoSWEET proteins into four subfamilies. Based on the phylogenetic tree, the gene structure, conserved motifs and cis-acting regulatory elements were analyzed further. Ten highly conserved motifs were identified, among which all the spinach SoSWEET proteins contained motifs 1, 2 and 4, constituting the most highly conserved part of spinach SoSWEET proteins. All members of the spinach SoSWEET proteins contained MtN3_slv and PQ-loop superfamily domains. The majority of SoSWEET protein family genes contain 5 introns, and the promoter contained cis-acting elements including light response, growth and development response, plant hormone response and stress response. The gene expression analysis showed that all SoSWEET genes were expressed in roots, stems, leaves and petioles, and downy mildew infecting. Collectively, These results provide an important reference for further studies on the functions of SoSWEET family genes in spinach. |
| Key words: spinach SoSWEET protein family downy mildew bioinformatics analysis |
