| 摘要: |
| 聚苯胺（PANI）是一种导电性良好、光电转化效率高的p型有机半导体.采用旋涂法制备了聚苯胺/氧化铟锡（PANI/ITO）电极，然后通过静电吸附将脲酶（urease）固定在PANI/ITO表面，形成Urease/PANI/ITO复合电极.利用脲酶分解尿素（urea）产生的氨可以改变PANI膜质子化状态，从而影响其光电性能的原理，发展可特异性检测尿素的光电化学（PEC）方法.该方法对尿素的线性检测范围较宽（10-3~10-8mol·L-1），特异性、稳定性和可重现性也较好，可用于人体尿液中尿素含量的测定. |
| 关键词: 聚苯胺(PANI) 光电化学(PEC) 脲酶(urease) 尿素(urea) |
| DOI：10.3969/J.ISSN.1000-5137.2022.05.007 |
| 分类号:O657.1 |
| 基金项目:国家自然科学基金（21976122）；上海晨光计划（17CG47） |
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| Development of a photoelectrochemical system based on polyaniline organic semiconductor for detection of urea |
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GU Xinxin, YANG Haifeng, WU Yiping
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College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China
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| Abstract: |
| Polyaniline(PANI) is a p-type of organic semiconductor with good conductivity and high photoelectric conversion efficiency. In this work, a polyaniline/indium tin oxide(PANI/ITO) electrode was prepared by spin coating. Then, the urease was immobilized on its surface by electrostatic adsorption to form an Urease/PANI/ITO composite electrode. The fixed urease on the surface catalyzes urea decomposition to generate ammonia, changing the PANI film's protonation state and further affecting its photoelectrochemical response. Based on this, a photoelectrochemical(PEC) method for the specific detection of urea was established. The technique has a broad linear detection range for urea(10-5-10-9 mol·L-1) and has reasonable specificity, stability, and reproducibility. It has been successfully used to detect urea content in human urine. |
| Key words: polyaniline(PANI) photoelectrochemistry(PEC) urease urea |
