| 摘要: |
| 癌症死亡患者中有90%由癌症转移引起，研究表明：患者的外周血、胸腔液等体液中的循环肿瘤细胞（CTC）与癌症转移及肿瘤结节转移（TNM）分期密切相关.因此，CTC检测在实体肿瘤前期诊断、预后及疗效评估等方面扮演着举足轻重的角色.该综述基于CTC区别于正常细胞的物理学、电学、生物学特征，总结了目前CTC分离富集及分析检测技术的研究进展，并就国内外CTC检测面临的挑战进行了讨论，对其未来发展趋势进行了展望. |
| 关键词: 循环肿瘤细胞（CTC） CTC检测 分离富集 |
| DOI：10.3969/J.ISSN.1000-5137.2020.02.011 |
| 分类号:O6-1;R730.4 |
| 基金项目:上海市地方院校能力建设专项（17070503000） |
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| Research progress on isolation, enrichment and detection of circulating tumor cells |
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PU Sisi, ZHOU Chaohui, JIA Nengqin
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College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China
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| Abstract: |
| Lots of studies showed that cancer metastasis is the main cause of 90% cancer mortality, and the number of circulating tumor cells(CTC)in body fluids such as peripheral blood and pleural fluid and so forth is closely related to the cancer metastasis and tumor node metastasis(TNM)staging. Therefore, CTC detection plays a pivotal role in preclinical diagnosis, prognosis, efficacy evaluation, and so on.This review summarizes the separation, enrichment and identification techniques for CTC detection based on the physical, dielectric and biological properties of CTC that are different from normal cells, and discusses the current progress and clinical application of CTC detection at home and abroad.In addition, the challenges and future prospect of CTC detection are discussed. |
| Key words: circulating tumor cells(CTC) CTC detection isolation and enrichment |
