| 摘要: |
| 胞外脱氧核糖核酸(eDNA)作为细菌生物膜胞外基质(EPS)的关键组成成分,在调控早期生物膜黏附和生长,以及维持成熟生物膜结构稳定等方面发挥着重要作用.近年来,研究表明,脱氧核糖核酸(DNA)结构会对生物膜的生长及结构性能产生影响. 然而,目前的研究主要集中于细菌自身基因组或者周围生命体释放的天然DNA分子对生物膜的作用,这类DNA多为长双链结构. 与之相比,短链DNA结构对生物膜的生长影响尚未可知.本研究设计并合成了包含16个碱基对的双链DNA(dsDNA)；采用结晶紫染色法,分析发现dsDNA对早期生物膜的生长影响,在不同发展时期和浓度条件下存在显著性差异；而激光共聚焦成像显示这些差异主要与dsDNA对细菌黏附及聚集体形成的影响相关.这些结果为深入理解DNA与生物膜之间相互作用提供了重要的理论依据. |
| 关键词: 短双链DNA 生物膜 可逆黏附期 不可逆黏附期. |
| DOI： |
| 分类号:Q 936 |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| Impact of Short Double-Stranded DNA on Biofilm Formation in the Initial Stages |
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ZHANG Fengjuan, ZHANG Wen, ZHAO Xinyi, MAO Ziyin, HANG Yundong, LYU Min
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College of Chemistry and Materials Science,Shanghai Normal University
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| Abstract: |
| Extracellular deoxyribonucleic acid (eDNA), as a key component of the biofilm extracellular polymeric substance (EPS), plays a pivotal role in regulating early biofilm adhesion and growth, as well as maintaining the structural stability of mature biofilms. Recent studies have shown that the structure of deoxyribonucleic acid (DNA) can significantly affect the growth dynamics and structural properties of biofilms. However, most existing research has focused on the effects of bacterial genomic DNA or naturally released DNA from surrounding organisms, which are typically long double-stranded. In contrast, how short double-stranded DNA affects biofilm development remains largely unexplored. Here, we designed and synthesized a 16-base-pair double-stranded DNA (dsDNA). The crystal violet assay revealed that the influence of dsDNA on early biofilm growth is dose-dependent across different stages of biofilm formation. Confocal imaging further indicated that these differences were primarily associated with dsDNA-induced bacterial aggregation during biofilm formation. These findings provide a solid theoretical basis for understanding the interactions between DNA and biofilm. |
| Key words: Short double-stranded DNA Biofilm Reversible adhesion Irreversible adhesion. |
