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贝莱斯芽孢杆菌S3-1与蚯蚓粪混合改良剂的制备及其土壤改良效果的研究
哈米古丽·吐尔逊江, 杨宁, 徐兆丰, 唐筱茸, 余雅婷, 田雅洁, 肖明
上海师范大学 生命科学学院, 上海 200234
摘要:
天然有机肥在提高土壤的肥力、促进作物生长等方面有着举足轻重的地位. 此前的研究中已发现菌株S3-1与蚯蚓粪共同施用时可以显著提高土壤肥力?. 本研究在此基础上,对实验样地、蚯蚓粪与商品菌肥进行了选择,根据比例制备改良剂. 关于改良剂对土壤环境因子的影响等问题,进行了长达270 d的原位修复实验. 实验样地分别为草坪地以及商品菌肥和蚯蚓粪处理地,设置了对照组、单独施加蚯蚓粪组、改良剂处理组、S3-1菌液处理组以及商品菌肥平行实验处理组. 结果发现:酸碱中性磷酸酶活性提升率依次为25.44%,20.32%和18.33%;真菌数量随着时间周期的增长率分别为9.92%,41.98%和51.65%,细菌数量的增长速率逐从20.70%~36.12%渐变大为36.12%~80.35%. 原位修复实验条件下,脲酶和蔗糖酶活性提升率也显著增强. 微生物改良剂用更安全更环保的方法,促进了土壤恢复到自然状态,本研究为改良剂的广泛使用性奠定了实践基础.
关键词:  微生物改良剂  原位改良  蚯蚓粪  贝莱斯芽孢杆菌  土壤酶活性
DOI:10.20192/j.cnki.JSHNU(NS).2024.04.008
分类号:Q93
基金项目:
Preparation of Bacillus velezensis S3-1 mixed with earthworm manure and study on soil improvement effect
HAMIGULI·TUERXUNJIANG, YANG Ning, XU Zhaofeng, TANG Xiaorong, YU Yating, TIAN Yajie, XIAO Ming
College of Life Sciences, Shanghai Normal University, Shanghai 200234, China
Abstract:
Natural organic fertilizer plays an important role in improving soil fertility and promoting crop growth. Previous research has found that strains the S3-1 with wormcast can significantly improve the soil fertility ? when applied. On this basis, the experimental plots, vermicompost and commercial bacterial fertilizer were selected, and the improver was prepared according to the proportion. About the effects of the amendments on soil environmental factors, in-situ remediation experiments were carried out for 270 d. The experimental plots were turf plots, commercial bacterial fertilizer plots and vermicompost treatment plots. Control group, vermicompost treatment group, amendment treatment group, S3-1 bacterial solution treatment group and commercial bacterial fertilizer parallel experimental treatment group were set up. The results showed that the increase rate of acid-base neutral phosphatase activity was 25.44%, 20.32% and 18.33%, respectively. The growth rate of fungus population was 9.92%, 41.98% and 51.65%, respectively, while the growth rate of bacteria population gradually increased from 20.70%-36.12% to 36.12%-80.35%. The activity of urease and sucrase increased significantly under the condition of in situ repair. Microbial amendments can promote the restoration of soil to its natural state in a safer and more environmentally friendly way. This study lays a practical foundation for the wide use of the amendments.
Key words:  microbial amendments  in-situ improvement  vermicompost  Bacillus velezensis  soil enzyme activity