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| 基于表型性状的菠菜核心种质构建 |
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彭枫1,2, 李阳3, 戴雨柔1,2, 王雪美1,2, 杨蛟1,2, 王全华1,2, 蔡晓锋1,2,3
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1.上海师范大学 生命科学学院, 上海 200234;2.上海师范大学 植物种质资源开发协同创新中心, 上海 200234;3.上海尚师农业科技有限公司 上海 201400
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| 摘要: |
| 以483份菠菜种质为材料，基于20个表型性状数据，根据2种遗传距离、3种取样方法、8种系统聚类方法、3种取样比例构建了144份菠菜核心种质.应用均值差异百分率、方差差异百分率、极差符合率和变异系数变化率4个参数来检验各取样策略的优劣.通过比较核心种质和原始种质的变异系数、方差、多样性指数、符合率和主成分，对构建的核心种质进行代表性检验.结果表明："欧氏距离+优先取样法+离差平方和法+15%的取样比例"取样策略最佳，其均值差异百分率、方差差异百分率、极差符合率和变异系数变化率分别为0，65%，100%和124.32%.核心种质与原始种质在20个指标上的均值符合率在95.08%~99.83%之间，最大值、最小值符合率均为100%，多样性指数符合率在83.94%~99.11%之间.原始种质和核心种质的前6个主成分相同，累积贡献率分别为69.563%和70.242%，表明所选出的72份菠菜核心种质能代表483份原始种质的表型变异特征性，有利于种质的保存和利用. |
| 关键词: 菠菜 核心种质 表型性状 种质资源 |
| DOI：10.3969/J.ISSN.1000-5137.2022.01.002 |
| 分类号:S636.1 |
| 基金项目:上海市科研创新行动计划项目（19070502600）；上海市科技兴农项目（沪农科推字（2021）第1-14号）；上海植物种质资源工程技术研究中心项目（17DZ2252700）；上海市协同创新中心项目（ZF1205）；上海市植物分子科学实验室项目（18DZ2260500） |
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| Construction of spinach's core germplasms based on its phenotypic traits |
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PENG Feng1,2, LI Yang3, DAI Yurou1,2, WANG Xuemei1,2, YANG Jiao1,2, WANG Quanhua1,2, CAI Xiaofeng1,2,3
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1.College of Life Sciences, Shanghai Normal University, Shanghai 200234, China;2.Development Center of Plant Germplasm Resources, Shanghai Normal University, Shanghai 200234, China;3.Shanghai Shangshi Agricultural Technology Co., Ltd., Shanghai 201400, China
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| Abstract: |
| This study used 483 spinach germplasm as materials, based on 20 phenotypic traits, and based on 2 genetic distances, 3 sampling methods, and 8 systematic clustering method and 3 sampling ratios constructed 144 core collections of spinach. Four parameters, the percentage of mean difference, the percentage of variance difference, the coincidence rate of range, and the rate of variation of the coefficient of variation, were used to test the pros and cons of each sampling strategy. By comparing the coefficient of variation, variance, diversity index, coincidence rate and principal components of the core collection and the original collection, the representative test of the constructed core collection was carried out. The results show that:"Euclidean distance+priority sampling method+deviation square sum method+15% sampling ratio" sampling strategy is the best, and its mean difference percentage, variance difference percentage, range coincidence rate, and variation coefficient change rate are 0, 65%, 100%, and 124.32% respectively. The average coincidence rate of the core collection and the original collection on the 20 indicators is between 95.08% and 99.83%, the maximum and minimum coincidence rates are both 100%, and the diversity index coincidence rate is between 83.94% and 99.11%. The first six principal components of the original germplasm and the core germplasm are same, and the cumulative contribution rates are 69.563% and 70.242%, respectively. It shows that the 72 spinach core collections constructed can represent the phenotypic variation characteristics of 483 original collections to be utilized and preserved. |
| Key words: spinach core germplasm phenotypic trait germplasm resource |
