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提高木质纤维素降解转化效率的初步探究
嵇朝球1, 许翊坤2, 虞睿睿2, 马中良2
1.江苏紫金有限公司, 江苏 扬州 225000;2.上海大学 生命科学学院, 上海 200444
摘要:
以农作物秸秆,包括水稻秸秆、玉米秸秆和甘蔗渣为材料,采用分光光度法比较了烟曲霉、绿色木霉、枯草芽孢杆菌的处理效果.实验结果显示:总乙醇产量最高的是甘蔗渣,其乙醇体积分数为6.285%;而玉米秸秆和水稻秸秆相差甚微,分别是6.200%和6.195%.用2%(体积分数)氢氧化钠溶液预处理甘蔗渣,再用烟曲霉在28℃下降解甘蔗渣,最后用缺陷型酵母发酵预处理滤液和烟曲霉降解液20~25 h,可以提高木质纤维素降解、转化乙醇的效率.
关键词:  秸秆  纤维素  乙醇  降解  效率
DOI:10.3969/J.ISSN.1000-5137.2021.04.015
分类号:Q939.97
基金项目:校企合作重大横向项目:高效微生物的开发及应用
Preliminary study on improving conversion efficiency of lignocellulose degradation
JI Chaoqiu1, XU Yikun2, YU Ruirui2, MA Zhongliang2
1.Jiangsu Zijin Limited Company, Yangzhou 225000, Jiangsu, China;2.School of Life Sciences, Shanghai University, Shanghai 200444, China
Abstract:
In this study, stems of crops, including straw of rice, stalk of corn and bagasse, were used as fermentation materials. The hydrolysis treatment effects of Aspergillus fumigatus, Trichoderma viride and Bacillus subtilis were compared via spectrophotometry. The results showed that the highest yield of ethanol was from bagasse with eventually volume fraction of ethanol in total was 6.285% volume fraction. The difference of yields between corn straw and rice straw was slightly, which were 6.200% and 6.195% respectively. Using 2% NaOH pretreating sugarcane bagasse, then using Aspergillus fumigatus to degradeat 28℃, 20-25 h fermentation of pretreated filtrate with defective yeast and Aspergillus fumigatus degradation solution, it can improve the degradation efficiency of the lignocellulose conversion.
Key words:  straw  cellulose  ethanol  degradation  efficiency