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| CRISPR技术对发酵乳制品中嗜酸乳杆菌的检测应用 |
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孟冬青, 李林显, 王金, 刘婷婷, 于靓, 俞漪, 刘洋, 赵磊, 郭敦爱, 蒋祯珍, 赵渝
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上海师范大学 生命科学学院, 上海 200234
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| 摘要: |
| 借助CRISPR-HOLMES系统，以市售酸奶作为检测对象，检测了其中的嗜酸乳菌含量.结果表明：CRISPR技术可作为一种有效直观的方法实现对发酵乳制品中嗜酸乳杆菌的定性检测. |
| 关键词: 乳制品 嗜酸乳杆菌 CRISPR 实时荧光定量PCR |
| DOI：10.3969/J.ISSN.1000-5137.2021.02.002 |
| 分类号:Q93.331 |
| 基金项目:上海市属高校应用型本科试点专业建设食品安全与检测（304-B-9101-19-001004）；上海市教育委员会第五批上海市属高校应用型本科试点专业建设（沪教委高〔2017〕79号） |
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| Application of CRISPR technology in the detection of Lactobacillus acidophilus in fermented dairy products |
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MENG Dongqing, LI Linxian, WANG Jin, LIU Tingting, YU Jing, YU Yi, LIU Yang, ZHAO Lei, GUO Dun'ai, JIANG Zhenzhen, ZHAO Yu
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College of Life Sciences, Shanghai Normal University, Shanghai 200234, China
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| Abstract: |
| In this paper,we detected Lactobacillus acidophilus content of some commercial brand yoghurts by using CRISPR-HOLMES system.The results showed that the CRISPR technology can be used as an effective and visual method to qualitatively detect Lactobacillus acidophilus in fermented dairy products. |
| Key words: dairy product Lactobacillus acidophilus CRISPR quantitative real-time PCR |
