| 摘要: |
| 采用电化学法在滴涂纳米氧化锌（ZnO）的玻碳电极（GCE）上聚合修饰1H-咪唑-4-甲酸（HIMC），制得聚1H-咪唑-4-甲酸-纳米ZnO（PHIMC-ZnO）复合膜修饰的GCE（PHIMC-ZnO/GCE），并用扫描电子显微镜（SEM）及电化学方法对修饰电极的形貌和电化学特性进行了表征.利用循环伏安法（CV）和差分脉冲伏安法（DPV）研究了抗坏血酸（AA）、多巴胺（DA）和尿酸（UA）在该修饰电极上的电化学行为.结果表明：在pH值为7.0的磷酸盐缓冲溶液（PBS）中，PHIMC-ZnO/GCE对AA，DA和UA均具有良好的电催化作用.在优化的实验条件下，AA，DA和UA的物质的量浓度线性范围分别为80~1 400，0.15~45.00和2~120 μmol·L-1，相关系数分别为0.998 0，0.993 7和0.998 3，检出限（S/N=3）分别为0.50，0.03和0.09 μmol·L-1.AA，DA和UA在不同扫速下的电化学行为表明：AA，DA和UA在PHIMC-ZnO/GCE上的电极过程受吸附过程控制，将PHIMC-ZnO/GCE应用于尿样和血清中AA，DA和UA的同时测定，结果令人满意. |
| 关键词: 1H-咪唑-4-甲酸（HIMC） 纳米氧化锌（ZnO） 抗坏血酸（AA） 多巴胺（DA） 尿酸（UA） |
| DOI：10.3969/J.ISSN.1000-5137.2020.02.009 |
| 分类号:O657.1 |
| 基金项目:上海市教委重点课程建设项目（A-0131-18-004031）；全国青年教师教育教学研究课题（2017QNJ079） |
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| Fabrication of poly(1H-imidazole-4-carboxylic acid)-zinc oxide nanoparticle modified glassy carbon electrode and its application for simultaneous determination of ascorbic acid, dopamine and uric acid |
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ZHAO Dan, ZHANG Lei
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College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China
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| Abstract: |
| A poly 1H-imidazole-4-carboxylic acid(PHIMC)modified glassy carbon electrode(GCE)pre-coated with zinc oxide (ZnO)nanoparticle(PHIMC-ZnO/GCE)had been fabricated and used for simultaneous detection of ascorbic acid(AA), dopamine(DA) and uric acid (UA). The surface morphology and electrochemical activity of the modified electrode were investigated by scanning electron microscopy(SEM)and electrochemical methods.The electrochemical behaviors of AA, DA and UA on the modified electrode were investigated by cyclic voltammetry(CV)and differential pulse voltammetry(DPV). The results show that modified electrode exhibits strong electro-catalytic activities toward oxidations of AA, DA and UA in a PBS buffer solution(pH=7.0).Under the optimum conditions, the linear concentration dependences of DPV current responses were observed for AA, DA and UA in the molar concentration ranges of 80-1 400, 0.15-45.00 and 2-120 μmol·L-1. The detection limits(S/N=3)of AA, DA and UA are 0.50, 0.03 and 0.09 μmol·L-1, respectively. And also, the different electrochemical behaviors of AA, DA and UA at PHIMC-ZnO/GCE under various scan rates indicate that the electrode reaction of AA, DA and UA is an adsorption-controlled process.The PHIMC-ZnO/GCE has been applied for the simultaneous determination of AA, DA and UA in human urine and serum samples with satisfactory results. |
| Key words: 1H-imidazole-4-carboxylic acid(HIMC) zinc oxide(ZnO)nanoparticle ascorbic acid(AA) dopamine(DA) uric acid(UA) |
