| 摘要: |
| 活性氧（ROS）介导的蛋白质半胱氨酸（Cys）可逆氧化修饰对于调控植物发育和逆境应答过程具有重要意义.近年来新发展起来的高通量氧化还原蛋白质组学技术包括：基于荧光素、生物素-巯基特异性试剂、同位素亲和标签（ICAT）、巯基特异性串联质量标签（CysTMT/iodoTMT），以及iodoTMT与同位素标记相对和绝对定量（iTRAQ）试剂联用等标记技术.这些技术的发展完善为系统研究植物蛋白质氧化还原修饰提供了重要手段.综述了植物Cys可逆氧化修饰蛋白质组学技术的研究进展. |
| 关键词: 植物 半胱氨酸 可逆氧化修饰 蛋白质组学技术 |
| DOI：10.3969/J.ISSN.1000-5137.2018.05.008 |
| 分类号:Q946.1 |
| 基金项目:中央高校基本科研业务费（2572016AA16，2572017ET01，2572017EA05） |
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| Advances on plant redox proteomics technologies for cysteine reversible oxidation |
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WANG Lin1, LI Ying1, DAI Shaojun2, YU Juanjuan1
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1.Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Harbin, Heilongjiang 150040, China;2.Development Center of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China
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| Abstract: |
| Reactive oxygen species (ROS)-mediated reversible oxidation of protein cysteine (Cys) plays an important role in the regulation of plant development and stress response. In recent years, high-throughput redox proteomics technologies have been developed, including several labeling strategies based on fluorescent reagents, biotin-conjugated alkylating reagents, isotope-coded affinity tags (ICAT), thiol-specific tandem mass tags (CysTMT/iodoTMT), as well as iodoTMT combined with isobaric tag for relative and absolute quantification (iTRAQ) reagents, respectively. The development of these technologies provides effective tools for systematically understanding the mechanisms of protein redox homeostasis in plants. In this review, the redox proteomics technologies applied in the research of plant Cys reversible oxidation have been summarized. |
| Key words: plant cysteine reversible oxidation modification proteomics |
