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转录物组学解析植物生长发育的生物钟调控模
孙亿敬, 上官燕, 王哲, 李姣爱, 刘祉辛, 孙旭武
上海师范大学 生命与环境科学学院, 上海 200234
摘要:
基于拟南芥的时间序列的基因组芯片数据,分析了植物生长的昼夜调节模式相关的基因表达规律,发现有2.4%的基因的日振幅达到了显著差异水平.从整体基因转录组水平分析,白天诱导表达的基因主要参与调控植物与环境之间的相互作用,而夜晚表达上调的基因主要参与调节植物的生长发育.此外,植物叶绿素和血红素的生物合成也受到了生物钟的调控.对整个基因组水平上生物钟核心震荡调节子CCA1/LHY和TOC1的共表达基因做了基因组水平上的扫描鉴定,得到了一些新的潜在的生物节律调节因子.这些结果为今后更为系统地完善植物的生物节律的调控网络提供了参考.
关键词:  转录物组学  植物  生长发育  生物钟
DOI:10.3969/J.ISSN.1000-5137.2018.04.008
分类号:Q756
基金项目:国家自然科学基金面上项目(31670233);上海植物种质资源工程技术研究中心项目(17DZ2252700)
Transcriptomic analysis of the circadian clock control pattern of plant growth and development
Sun Yijing, Shangguan Yan, Wang Zhe, Li Jiaoai, Liu Zhixin, Sun Xuwu
College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China
Abstract:
Based on the time series genomic microarray data of Arabidopsis thaliana,the gene expression pattern was analyzed,and it was found that 2.4% of the genes had a significant difference in daily amplitude.From the analysis of the overall gene transcriptome level,the genes induced during the day are mainly involved in the regulation of the interaction between plants and the environment,while the genes whose expression is up-regulated at night are mainly involved in regulating the growth and development of plants.In addition,the biosynthesis of plant chlorophyll and hemeare also regulated by the circadian clock.A group of new potential biorhythm regulators were identified by analyzing the genes that show co-expressing with CCA1/LHY or TOC1.These data will provide a reference for the more systematic improvement of the regulatory network of plant circadian clock.
Key words:  transcriptomic  plant  growth and development  circadian clock