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| 毒死蜱降解菌CD7的筛选与植物根际促生菌JD37的联合应用 |
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苏翠珠1, 蒋秋悦1,2, 章丽1, 罗斯1, 杨文武1, 孙舒荣1, 肖明1
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1.上海师范大学 生命与环境科学学院 植物种质资源开发协同创新中心, 上海 200234;2.复旦大学 生命科学学院, 上海 200438
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| 摘要: |
| 筛得一株毒死蜱降解菌CD7，联合植物根际促生菌JD37，研制了一种能修复土壤的农药污染，同时促进植物生长的复合型土壤改良剂.实验结果显示：将CD7和JD37发酵液按体积比1：1使用，可以促进植物生长，并在25 d内降解土壤中约66.43%的毒死蜱.在载体量相同情况下，蚯蚓粪比滑石粉吸附更多混合菌株.在室温贮藏一个月，活菌数为4.81×107 CFU/g；制备的复合改良剂与土壤按质量比1：1使用时，能促进植物生长、提升土壤酶活性、增加土壤中微生物的数量. |
| 关键词: 植物根际促生菌 毒死蜱降解菌 CD7 土壤改良 筛选 |
| DOI：10.3969/J.ISSN.100-5137.2017.02.022 |
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| 基金项目:上海市科委重点项目（16391902100） |
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| Screening of chlorpyrifos degrading bacteria CD7 and its combined application with PGPR JD37 |
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Su Cuizhu1, Jiang Qiuyue1,2, Zhang Li1, Luo Si1, Yang Wenwu1, Sun Shurong1, Xiao Ming1
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1.Development Center of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;2.School of Life Sciences, Fudan University, Shanghai 200438, China
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| Abstract: |
| We screened a chlorpyrifos degrading bacteria,Burkholderiasp. CD7.Joint with plant growth-promoting rhizobacteria(PGPR) JD37 to produce a compositesoil amendment,which could restorethe pesticides polluted soil and promote plant growth.Results showed that CD7 and JD37 (at the volume ratio of 1:1) can promote the growth of plants,and within 25 days degrade about 66.43% chlorpyrifos in the soil.Further research found that under the same conditions of carrier dosage,vermicompost can adsorbed more bacteria than talcum powder;after a month preservation at room temperature,the number of living bacterium still maintained about 4.81×107 CFU/g.Carrier and soil,at the mass ratio of 1:1,could optimally promote plant growth,improve soil enzyme activities and increase the number of microorganisms in soil. |
| Key words: plant growth-promoting rhizobacteria chlorpyrifos degrading bacteria CD7 soil amendment screening |
