| 摘要: |
| 通过对白发藓生长最佳基质和营养液的筛选,来大量纯种扩繁白发藓.通过组织培养得到了白发藓(Leucobryumglaucum)的无菌配子体,将它们接种于装有泥炭土、蛭石和珍珠岩不同基质比例的培养瓶内,并加入不同浓度的knop′s营养液,通过60 d的培养,测定了不同处理的白发藓配子体的叶绿素含量,配子体长度、总鲜重和分枝数等指标.发现泥炭土∶蛭石∶珍珠岩2∶2∶1比例、20%浓度的Knop′s营养液有利于白发藓配子体的生长. |
| 关键词: 白发藓 离体培养 泥炭土 营养液 |
| DOI： |
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| 基金项目:上海市科委(11391901200,12490502700);上海市高校知识服务平台-上海市植物种质资源中心经费 |
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| On influences of cultivated media and nutrient concentrations on growth of leucobryum glaucum |
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DING Xue, GUO Shuiliang, LOU Yuxia
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Development Center of Plant Germplasm Resources,College of Life and Environmental Sciences,Shanghai Normal University
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| Abstract: |
| Screening by Leucobryum glaucum growth optimal substrate and nutrient solution,to a large number of purebred breeding leucobryum.The flask culture conditions of the gametophyte of Leucobryum glaucum inoculated with peat,vermiculite and perlite matrix at different proportions,and adding knop′s nutrition liquid at different concentrations.After a 60-day cultivation,the chlorophyll content,length,total fresh weight and branch number of the gametophytes of L.glaucum were measured.The results showed that L.glaucum could grow well on the medium with a proportion of peat,vermiculite and perlite at 2∶2∶1,together with 20% Knop′s nutrition liquid. |
| Key words: glaucum invitro peat nutrition |
