| 摘要: |
| TDF1作为MYB家族的转录因子,参与了胼胝质的降解过程,并控制绒毡层的分化从而影响着花粉粒的正常发育.以一株通过甲基磺酸乙酯(EMS)诱变Col-0野生型拟南芥获得的温敏雄性不育突变体atms1(ambient temperature-sensory male sterility1)对温度敏感机制进行了研究.通过构建TDF1反义RNA表达载体,在atms1突变体背景下抑制TDF1的表达来观察突变体花粉育性的变化,发现在27℃时的atms1突变体花粉的育性得以恢复.这一结果表明在花粉发育的过程中TDF1对ATMS1m具有一定的调控作用. |
| 关键词: atms1 温敏雄性不育 TDF1 花药育性 Real-time PCR |
| DOI： |
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| 基金项目:国家自然科学基金面上项目(30870225);上海市教委浦汇人才计划(08PJLH05500) |
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| Reaserch on temperature-sensitive regulation mechanism of transcription factor TDF1 to thermo-sensitive male sterile mutant atms1 of arabidopsis thaliana |
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ZHENG Yajie, GAO Bei, XIA Qunfang, ZHOU Shumin, ZHANG Wei
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Shanghai Key Laboratory of Bio-Energy Crops,School of Life Sciences,Shanghai University
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| Abstract: |
| TDF1 is a transcription factor of MYB family.It is involved in the process of callose degradation and regulating the tapetum differentiation,thus affecting the normal development of pollen.This paper investigates the temperature-sensitive mechanism of an Arabidopsis male sterile mutant atms1 (ambient temperature-sensory male steility1) obtained by screening in the progeny generations of wild-type Col-0 mutagenized by EMS. TDF1 Antisense RNA vector was introduced into atms1 background to inhibit the expression of TDF1 to observe the changes of atms1 mutant pollen fertility.We found that the fertility was restored at 27 ℃.The results show that TDF1 may have regulatory effects on ATMS1m in the process of pollen development. |
| Key words: atms1 temperature-sensory male sterile TDF1 anther fertility Real-time PCR |
