| 摘要: |
| MADS-box转录因子,作为一个大的基因家族,在植物生长发育过程中起重要作用,尤其是作为开花植物花器官形成的主要调控者.该基因家族在揭示进化方面也有着重大意义.为了探究MADS-box基因的进化,需要更多的非开花植物中MADS-box基因的信息.选取水蕨(Ceratopteris thalictroides)作为材料,用RACE方法对其MADS-box基因进行克隆与分析.在水蕨中克隆得到两条MADS-box基因,分别命名为CtMADS1和CtMADS2.分析显示:CtMADS1属于MIKC*分支,而CtMADS2属于MIKCc分支,水蕨的这2条MADS-box基因与开花植物的MADS-box基因有着很近的亲缘关系,现在维管植物最近的共同祖先中至少存在2条不同的花同源异形基因,即MADS-box基因. |
| 关键词: 水蕨 演化发育生物学 MADS-box基因 系统进化分析 |
| DOI： |
| 分类号: |
| 基金项目:上海师范大学校理科科研基金(SK201231) |
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| Cloning and analysis of two Ceratopteris thalictroides MADS-box genes |
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XU Daolan, DAI Xiling, XU Ying, WANG Quanxi
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College of Life and Environmental Sciences,Shanghai Normal University
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| Abstract: |
| MADS-box transcription factors,as a large gene family,play an important role in plant growth and development,especially act as key regulators in controlling the identities of floral organs in flowering plants.They are also significant in the evolutionary revelation.In order to understand MADS-box genes,we need more information of MADS-box genes in non flowering plant.MADS-box genes of Ceratopteris thalictroides were selected to clone and analysis by using RACE method.Two MADS-box genes,designated CtMADS1 and CtMADS2 in C. thalictroides,were cloned.Analysis indicates that CtMADS1 is belonged to MIKC*-clade,while CtMADS2 is belonged to MIKCc-clade.Phylogeny suggests that these two MADS-box genes of C. thalictroides have a close relationship with flowering plants,the data indicates that at least two different MADS-box genes are homologous to floral homeotic genes existed in the last common ancestor of contemporary vascular plants. |
| Key words: Ceratopteris thalictroides evo-devo MADS-box gene phylogenetic analysis |
