| 摘要: |
| 研究了四环素耐药菌和四环素抗性基因在污泥深度脱水中的去除特性,分别采用稀释平板涂布法和荧光定量PCR对污泥深度脱水前后四环素耐药菌和4种四环素抗性基因(tetA、tetB、tetM和tetX)进行了测定,分析了污泥脱水前后这些污染物的去除特性.结果表明:污泥深度脱水前后四环素耐药菌和四环素耐药基因均被检出,经污泥深度处理工艺处理后,四环素耐药菌和所测定的四环素耐药基因均得到一定去除,tetA、tetB和tetM的去除率均达到80%以上.论文结果可为耐药菌和耐药基因的污染控制提供参考. |
| 关键词: 污泥 深度脱水 四环素耐药基因 四环素耐药菌 去除特性 |
| DOI： |
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| 基金项目:国家自然科学基金(21007010)；湖南省交通厅科研项目(200908,201105)；交通运输部科研项目(010353343290) |
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| Removal characteristics of tetracyclines resistant bacteria and tetracycline resistance genes in sludge deep dehydration |
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TAN Guogan1, DU Yongli2, HUANG Manhong3, ZHANG Wei3
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1.Civil Engineering Co.,Ltd of China Construction Fifth Engineering Bureau;2.Hunan Provincial Communication Planning,Survey & Design Institute;3.College of Environmental Science and Engineering,Donghua University
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| Abstract: |
| In order to investigate the removal characteristics of Tetracyclines Resistant Bacteria (TRB) and Tetracycline Resistance Genes (TRGs) in sludge deep dehydration,Dilution plate coating method and Real-Time Polymerase chain reaction (RT-PCR) were used to detected the numbers of TRB and four tet genes (tetA、tetB、tetM and tetX) abundant before and after sludge dehydration,and then TRB and TRGs removal characteristics before and after sludge deep dehydration were analyzed.Results showed that:both TRB and TRGs were determined before and after sludge deep dehydration.However,the TRB and TRGs were partly removed after the treatment of sludge deep dehydration.tetA, tetB and tetM removal percentages reached more than 80%.The results can be used as reference for the control of TRB and TRGs contamination. |
| Key words: sludge deep dehydration tetracycline resistant genes tetracycline resistant bacterium removal characteristics |
