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利用分子标记分析22种上海市重要水稻10个食味品质相关基因的基因型
谢米雪1, 郭亮1, 窦兰兰1, 曹月琴2, 李建粤1
1.上海师范大学;2.上海市闵行区农业科学研究所
摘要:
稻米食味品质改良是目前水稻育种的重要研究方向之一.明确不同水稻食味品质基因型,可为优质水稻育种研究的亲本选择提供依据.本研究利用分子标记检测技术,对22个目前上海市种植的重要常规水稻和杂交稻亲本食味品质基因的基因型进行检测.结果发现,“紫香糯861”水稻含有相对较多不良食味品质基因型.3种杂交稻父本,“湘晴”、“R44”和“繁14”的食味品质基因型比这3种杂交稻母本,“寒风A”、“秋风A”和“申9A”以及其他常规稻有欠缺.从蜡质基因多态性分析显示,在本研究选用的22种水稻中,2个为糯稻、20个为粳稻,但其中有4个为软米型粳稻;在16个非软米型粳稻中,“宝农34”、“金丰”和“银香18”是具有相对最好食味品质基因型的水稻品种.
关键词:  水稻  食味品质基因  基因型  分子标记
DOI:
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基金项目:上海市科委项目(10391900603)
Genotypes analysis of ten genes related to eating quality of 22 Shanghai important rice varieties by molecular marker
XIE Mixue1, GUO Liang1, DOU Lanlan1, CAO Yueqin2, LI Jianyue1
1.Development Center of Plant Germplasm Resources,College of Life and Environment Sciences,;2.Institute of Agricultural Sciences of Minhang District
Abstract:
The improvement of rice eating quality is a very important direction of rice breeding.Clearing the different genotype of rice eating quality provides the basis for the parent selection on breeding good eating quality rice.Using molecular marker detection technology,genotypes of ten genes related to eating quality of 22 Shanghai important rice varieties,including conventional rice and hybrid parents were detected.The results showed that "Zixiangnuo 861" rice contained many poor genotypes which might affect rice eating quality.The genotypes of 3 male parents,"Xiangqin","R44" and "Fan14" for three kinds of hybrid rice,"Hannyouxiangqing","Qiuyoujinfeng" and "Huayou14",respectively,were not as good as their corresponding female parents,"HanfengA","JinfengA" and "Shen9A" and another conventional rice varieties.Wx polymorphism analysis showed that there were two glutinous rice,four soft rice japonica and 16 non-soft rice japonica."Baonong34","Jinfeng" and "Yinxiang18" had relatively better genotypes of the genes controlling eating quality among the 16 non-soft rice japonica.
Key words:  rice  eating quality gene  genotype  molecular marker