| 摘要: |
| 在60Coγ射线辐照的水稻突变体库中,发现了一个以粳稻品种日本晴为遗传背景的幼苗叶色黄化突变体syl11(seedling yellow leaf 11).与野生型相比,突变体幼苗第二和第三叶表现黄色,在其完全展开之前叶片自其顶端开始转绿,长到四叶期其叶色恢复正常;并且该突变体syl11幼苗黄色叶片光合色素含量明显下降.遗传分析表明,该突变体的遗传性状由1对隐性核基因控制.本研究以培矮64S/syl11的F2代突变型植株作为定位群体,应用微卫星(SSR)分子标记以及新发展的InDel分子标记,将基因syl11定位在水稻第11号染色体长臂上的RM26652和处于着丝粒附近的ID11974分子标记之间,其遗传距离分别为0.5 cM和0.7 cM. |
| 关键词: 水稻 光合色素 叶色突变体 基因定位 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金(30971552);上海市科委项目(10DZ2271800,12ZR1422000,09DJ1400505,0939191200);上海市教委项目(J50401) |
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| Genetic analysis and preliminary gene mapping of a novel seeding yellow leaf mutant in rice |
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GE Shaoxing, MA Xiaojing, LIN Dongzhi, DONG Yanjun
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College of Life and Environment Sciences, Shanghai Normal University
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| Abstract: |
| A novel seeding yellow-leaf mutant, syl11(seedling yellow leaf 11),was found in rice mutant pool from japonica variety,Nipponbare,treated by 60Coγ-radiation.In comparison with the wild-type parent (Nipponbare),the mutant displayed the phenotype of yellow on the 2nd and 3rd leaf and began to turn green from its top before they completely expanded besides the significantly decreased contents of photosynthetic pigments,but all leaves become normal green after the 4-leaf-old stage.The genetic analysis indicated that the mutant trait was controlled by a single recessive nuclear gene.In addition,with the help of SSR,new developed InDel markers and F2 segregating population derived from the cross between the syl11 mutant and Peiai64S,the syl11 was mapped between RM26652(on the long arm)and ID11974(closing to the centromere)on chromosome 11,with a genetic distance of 0.5cM and 0.7cM,respectively. |
| Key words: rice(Oryza sativa) photosynthetic pigments leaf-color mutant gene mapping |
